KMID : 0848120170420010001
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International Journal of Oral Biology 2017 Volume.42 No. 1 p.1 ~ p.8
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Peripheral Cellular Mechanisms of Artemin-induced Thermal Hyperalgesia in Rats
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Kim Hye-Jin
Yang Kui-Ye Lee Min-Kyung Park Min-Kyoung Son Jo-Young Ju Jin-Sook Ahn Dong-Kuk
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Abstract
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In the present study, we investigated the role of peripheral ionotropic receptors in artemin-induced thermal hyperalgesia in the orofacial area. Male Sprague-Dawley rats weighting 230 to 280 g were used in the study. Under anesthesia, a polyethylene tube was implanted in the subcutaneous area of the vibrissa pad, which enabled drug-injection. After subcutaneous injection of artemin, changes in air-puff thresholds and head withdrawal latency time were evaluated. Subcutaneous injection of artemin (0.5 or 1 ¥ìg) produced significant thermal hyperalgesia in a dose-dependent manner. However, subcutaneous injection of artemin showed no effect on air-puff thresholds. IRTX (4 ¥ìg), a TRPV1 receptor antagonist, D-AP5 (40 or 80 ¥ìg), an NMDA receptor antagonist, or NBQX (20 or 40 ¥ìg), an AMPA receptor antagonist, was injected subcutaneously 10 min prior to the artemin injection. Pretreatment with IRTX and D-AP5 significantly inhibited the artemin-induced thermal hyperalgesia. In contrast, pretreatment with both doses of NBQX showed no effect on artemin-induced thermal hyperalgesia. Moreover, pretreatment with H-89, a PKA inhibitor, and chelerythrine, a PKC inhibitor, decreased the artemin-induced thermal hyperalgesia. These results suggested that artemin-induced thermal hyperalgesia is mediated by the sensitized peripheral TRPV1 and NMDA receptor via activation of protein kinases.
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KEYWORD
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artemin , thermal hyperalgesia , peripheral ionotropic receptor , TRPV1 , NMDA
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